Improvement of the Digestibility of Sulfated Hyaluronans by Bovine Testicular Hyaluronidase: A UV Spectroscopic and Mass Spectrometric Study
Negative ion MALDI-TOF mass spectrum of the sulfated HA with dss = 3.0 subsequent to digestion with BTH at optimized conditions but without further purification of the reaction mixture. All spectra were recorded in the linear mode of the MS device and in the presence of 9-aminoacridine as matrix using a sample to matrix ratio of 2 to 1 (v/v). Sulfated tetrasaccharides are marked by grey and hexasaccharides by white bars, respectively. Obvious impurities are marked by asterisks.