Huangqin-Tang Ameliorates TNBS-Induced Colitis by Regulating Effector and Regulatory CD4⁺ T Cells

Figure 5

HQT regulates protein levels of Th1-, Th2-, Th17-, and Treg-related cytokines in the TNBS-induced colitis model. Rats with TNBS-induced colitis were treated with or without HQT (120 mg/kg). Mesenteric lymph nodes (MLNs) cells from each group were stimulated with anti-CD3/CD28 antibodies and the cultural supernatants were harvested, followed by ELISA analysis of cytokines indicated above (pg/mL). Th1-related cytokines (a) IFN-γ and (b) IL-12; Th2-related cytokines (c) IL-4, (d) IL-13, and (e) IL-5; Th17-related cytokines (f) IL-17A and (g) IL-6; Treg-related cytokines (h) IL-10 were measured. Results represent the mean ± SD from eight mice per group. , versus the control group. ^Δ, ^ΔΔ versus TNBS-treated rats.

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