Astroglia-Microglia Cross Talk during Neurodegeneration in the Rat Hippocampus
Timing of the microglial reaction to NMDA in the hippocampus. Photomicrographs illustrate the hippocampal injury and microglial reaction to NMDA-induced lesion at the injection site. Photomicrographs of cresyl-violet stained brain sections of (a) sham rats and (b) NMDA rats 15 days after the lesion. (c) Graph shows the quantification of the area of lesion relative to the whole HF in cresyl-violet stained sections. IB4 histochemistry of sham (PBS) (d) and NMDA rats (e) 15 days after the lesion. (f) Distribution of specific binding sites for [3H]PK-11195 in a coronal rat brain section 15 days after 20 nmol NMDA injection. Note the NMDA-induced increase of specific binding (arrowhead) seen as a white area in the left hippocampus. Graphs show the quantification of the area of microglial reaction in IB4-stained sections (g), the area of enhanced [3H]PK-11195 specific binding (h), and the hippocampal concentration of TNF-α (i) of PBS and NMDA rats during the 38 days of the study. Asterisks in (a), (b), (d), and (e) indicate the injection site. CA1, Cornu Ammonis area 1; DG, dentate gyrus. different from PBS; # different from day 0 (LSD post hoc test in (d), (f); KW test in (e)) ( PBS; NMDA rats). Bar: 1 mm in (a), (b), (d), and (e) and 2 mm in (f).
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