Astroglia-Microglia Cross Talk during Neurodegeneration in the Rat Hippocampus
α-AA effect on the NMDA-induced microglial reaction in the hippocampus. (a) Quantification of the hippocampal area of microglial reaction in IB4-stained sections of sham (PBS), α-AA, NMDA, and α-AA + NMDA rats at postlesion days 1, 3, 15, and 38. Photomicrographs show hippocampal IB4-stained cells illustrating different microglial morphology between NMDA (b) and α-AA + NMDA (c) rats 3 days after the lesion. (d) TNF-α hippocampal concentration in PBS, α-AA, NMDA, and α-AA + NMDA rats during the first 5 days of the study. (e) Immunoblots and densitometric analysis (graphs) of YM1 in the hippocampus of PBS, α-AA, NMDA, and α-AA + NMDA rats during the first 5 days of the study. Values were normalized to β-actin bands. (f) Quantification of the nucleus/cytoplasm ratio of GR-immunolabeling in hippocampal glial cells 3 days after the lesion of the four groups of the study. P, PBS; A, α-AA; N, NMDA; NA, α-AA + NMDA; different from PBS; # different from NMDA; different from α-AA (LSD post hoc test in (a), (e); Student’s -test in (d), (f)) ( rats/group). Bar: 10 μm.
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