Early-Onset Diabetic E1-DN Mice Develop Albuminuria and Glomerular Injury Typical of Diabetic Nephropathy

Figure 2

E1-DN mice present mesangial expansion and glomerular sclerosis. (a) Periodic acid-Schiff (PAS) staining of normal glomerulus of the wild-type mouse aged 50 weeks. ((b), (c)) Global mesangial expansion (arrows) is observed by PAS staining in the homozygous E1-DN mice aged 20 weeks (b) and 40 weeks (c). (d) Global nodular sclerosis (arrowheads) is seen in an E1-DN homozygous mouse aged 50 weeks. (e) The percentage of mesangial area in the glomerular tuft determined by morphometry of PAS-stained histological sections indicates an increase of 25% in the homozygous (hoz) E1-DN mice when compared to wild-type (wt) mice ( glomeruli from three 50 weeks old wild-type mice and glomeruli from seven 20–50 weeks old homozygous mice). (f) Morphometric analysis performed on images obtained by transmission electron microscopy (TEM) confirms the increase of mesangial volume fraction by 22% in the glomeruli of E1-DN homozygous mice ( glomeruli from two 50 weeks old wild-type mice and glomeruli from six 20–50 weeks old homozygous mice). In ((e), (f)), bars show mean ± SEM. , , Student’s -test. Scale bar: 25 μm.

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