Research Article

Early-Onset Diabetic E1-DN Mice Develop Albuminuria and Glomerular Injury Typical of Diabetic Nephropathy

Figure 4

E1-DN mice show increased podocyte apoptosis. ((a), (b)) Staining for cleaved caspase-3 was used to identify apoptotic cells (arrows) in the glomeruli of wild-type (a) and homozygous E1-DN mice (b). (c) Apoptosis of glomerular cells is detected more often in the homozygous (hoz) E1-DN mice when compared to heterozygous (hez) and wild-type (wt) mice. Bars show the mean number of apoptotic cells per 10 glomeruli ± SEM, mice/group. , Mann-Whitney test. (d) Confocal microscopy of kidney sections of 20 weeks old homozygous E1-DN mice stained for nephrin (green) and cleaved caspase-3 (red) indicates that the apoptotic cells in the glomeruli are podocytes. Scale bar: 25 μm.
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