Next-Generation Technologies for Multiomics Approaches Including Interactome Sequencing
Table 3
Comparison of major metabolomics methods.
Method
Benefit
Drawback
NMR
(1) Results obtained in a single experiment (2) Fast (3) Available for studying various nuclei (4) Consistent with liquid and solid matrices (5) Quantitative (6) Sample can be recovered after analysis
(1) Requires highly skilled technicians and statisticians (2) Limited sensitivity (1–10 µmol/L) (3) Coresonant metabolites can be challenging to quantify
GC-MS
(1) High accuracy and repeatability of results (2) Small amounts of samples are required (3) High discrimination between molecules exhibiting very similar structures (4) High sensitivity (1 pmol/L)
(1) Sample preparation (including derivatization) can be time-consuming (2) Not all compounds are suitable for gas chromatography
LC-MS
(1) Wide application (2) High sensitivity (1 pmol/L)
(1) Requires extensive sample preparation, including derivatization (2) Long analytical time (20–60 min per sample) (3) Limited to volatile compounds (4) Suffers from ion suppression
CE-MS
(1) Suitable for polar molecules (2) Large separation capacity (3) High sensitivity (1 pmol/L)
