Next-Generation Technologies for Multiomics Approaches Including Interactome Sequencing

Table 4

Comparison of comprehensive protein-protein interaction analysis methods.

Method	Selection condition of PPIs	Library size	Cell cloning required	Next-generation sequencing
Y2H	In vivo	10^6	Yes	Applicable but limited
TAP-MS	In vitro	Living body sample	Yes	Inapplicable
Protein microarray	In vitro	Living body sample	No	Inapplicable
Shotgun proteomics	In vitro	Living body sample	No	Inapplicable
IVV	In vitro	10^12	No	Applicable and effective

See also Table 1.
Y2H: yeast two-hybrid; TAP-MS: tandem affinity purification-mass spectrometry; IVV: in vitro virus.