The paper titled “Toxicity of Graphene Shells, Graphene Oxide, and Graphene Oxide Paper Evaluated with Escherichia coli Biotests” [1] contains textual errors we wish to correct. The corrections are as follows.
In Section “2.1. Graphene-Family Nanomaterials” the sentence “Aqueous suspensions of GS and GO (from 10−3 to 2 × 10−5 mg/L) …” has numerical errors. The correct sentence is as follows: “… (from 1000 to 20 mg/L)….”
In Section “2.6. Efflux of Intracellular DNA Content” the sentence “The plasmid-containing E. coli K12 TG1 lac::lux strain was cultured in mid-exponential growth phase with ampicillin and then separated from its cultivation medium and incubated with aqueous GO suspensions at concentrations of 1.25 × 10−4, 5 × 10−4, and 2 × 10−3 mg/L for 60 min, …” has numerical errors. The correct sentence is as follows: “… at concentrations of 125, 500, and 2000 mg/L….”
In Section “2.7. Zeta Potential Measurement” the sentence “A bacterial suspension (3.7 × 108 CFU/mL) was mixed with serially diluted GO aqueous suspensions to a final concentration from 3.1 × 10−5 to 5 × 10−4 mg/L” has numerical errors. The correct sentence is as follows: “… to a final concentration from 31.25 to 500 mg/L.”
In Section “3.1.1. GFN Toxicity Assessment Using a Bacterial Bioluminescence Inhibition Assay” the sentence “In the current system containing 3.7 × 108 CFU/mL cells, GO toxicity was characterized by EC50 values of 1.13 ± 0.05 × 10−4, 1.04 ± 0.03 × 10−4, and 7.92 ± 0.24 × 10−5 mg/L …” has numerical errors. The correct sentence is as follows: “… by EC50 values of 113.1 ± 4.52, 104.2 ± 3.12, and 79.2 ± 2.37 mg/L….”
The sentence “At the 60th min of measurement these values varied from 1.13 ± 0.05 × 10−4 to 2.55 ± 0.10 × 10−5 mg/L …” has numerical errors. The correct sentence is as follows: “… values varied from 113.1 ± 4.52 to 25.5 ± 1.01 mg/L….”
In Figure legend designations have numerical errors: “1: 10−3 mg/L; 2: 5 × 10−4 mg/L; 3: 2.5 × 10−4 mg/L; 4: 1.25 × 10−4 mg/L; 5: 6.25 × 10−5 mg/L; 6: 3.13 × 10−5 mg/L; 7: 1.56 × 10−5 mg/L; 8: 7.81 × 10−6 mg/L; 9: 3.91 × 10−6 mg/L; C: control; wp: white paper, bp: black paper, GO-P: graphene oxide paper.”
The correct designations are as follows: “1: 1000 mg/L; 2: 500 mg/L; 3: 250 mg/L; 4: 125 mg/L; 5: 62.5 mg/L; 6: 31.25 mg/L; 7: 15.62 mg/L; 8: 7.81 mg/L; 9: 3.91 mg/L; C: control; wp: white paper; bp: black paper; GO-P: graphene oxide paper.”
The -axis values of GO in Figure should be corrected to the following: from 0 to 250 mg/L.
In Table the values of EC50 (mg/L content) should be replaced by those shown in the corrected table.
In Section “3.1.2. GFN Bioactivity in a Fluorescent Cell Assay” the sentence “In contrast, bacterial cells treated with GO suspensions became red-fluorescent after 60 min in a dose-dependent manner, from 48.8 ± 1.9% (at 1.25 × 10−4 mg/L GO) to 93.0 ± 2.8% (at 5 × 10−4 mg/L GO) …” has numerical errors. The correct sentence is as follows: “… from 48.8 ± 1.9% (at 125 mg/L GO) to 93.0 ± 2.8% (at 500 mg/L GO)….”
In Figure legend, the E. coli K12 TG1 cells were treated with graphene shells suspensions at 1000 mg/L instead of 10−3 mg/L and treated with graphene oxide suspension at 125 mg/L instead of 1.25 × 10−4 mg/L.
In Section “3.2.1. GO Validation as a Potential Oxidative Stress Inducer in Lux-Biotests” the sentence “The 1.2–1.5-fold light intensity growth only has been recorded at subtoxic GO concentrations (1.95 × 10−6–10−3 mg/L)” has numerical errors. The correct sentence is as follows: “… at subtoxic GO concentrations (1.95–1000 mg/L).”
The sentence “In turn, GO concentrations of 2 × 10−3 mg/L or more were toxic …” should be corrected to the following: “In turn, GO concentrations of 2000 mg/L or more were toxic….”
In Section “3.2.2 Intracellular DNA Efflux Measurement in GO-Treated Escherichia coli Cells” the sentence “In turn, DNA levels in supernatants of cells exposed to GO at concentrations of 1.25 × 10−4–2 × 10−3 mg/L …” should be corrected to the following: “… 125–2000 mg/L….”
In Section “3.2.3. Effect of GO Treatment on Zeta Potential Properties of Escherichia coli Cells” the sentence “Finally, the GO concentration required for complete zeta potential neutralization was 2.50 × 10−4 mg/L, whereas concentrations in the range of 3.13 × 10−5–1.25 × 10−4 mg/L …” has numerical errors. The correct values are “… 250 mg/L …” and “… 31.25–125 mg/L …,” respectively.
The -axis values of GO in Figure should be corrected to the following: from 0 to 500 mg/L.
These corrections do not influence the results, discussion, or conclusion of this work.
