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Figure 5: EE attenuated sevoflurane-induced cognitive impairment in 6-day-old mice. (a) Sevoflurane downregulated the protein levels of PPAR-γ, and exposure to environmental enrichment (EE) for 2 h daily from P8 to P30 attenuated this decrease in PPAR-γ levels. (b) Quantification of western blots confirmed that EE attenuated the sevoflurane-induced decrease in the protein levels of PPAR-γ (). (c) EE did not increase the escape latency of mice in the MWM, as compared to the control condition (tested from P30 to P34) (control, ; EE, ). (d) EE had no effect on the platform crossing times of mice at P34, as compared to the standard environment (SE) (control: ; EE: ). (e) There was a statistically significant interaction of time and group, based on the escape latency of the MWM, between mice that were exposed to sevoflurane and mice that were exposed to both sevoflurane and EE (two-way ANOVA; sevoflurane: ; sevoflurane + EE: ). (f) The Mann-Whitney test showed that there was a significant difference in the platform crossing times between mice that were exposed to sevoflurane and mice that were exposed to both sevoflurane and EE (sevoflurane: ; sevoflurane + EE: ).