Proteomic Study to Survey the CIGB-552 Antitumor Effect

Figure 1

Chemical proteomic approach (a) and results (b). (a) The streptavidin-sepharose (grey) biotinylated CIGB-552 complex retains the drug targets (green) and secondary binders (orange). Nonspecific proteins (blue) stick to the matrix. The retained proteins were eluted, separated by SDS-PAGE, and identified by LC-MS/MS. (b) Proteins eluted from the streptavidin-sepharose matrix were separated by SDS-PAGE. Molecular weight markers (MWM) displayed on the left. Lane I: protein cell extract (Hep-2 cell line); lane II: proteins eluted from the unloaded matrix (without CIGB-552-B); lane III: proteins eluted from the matrix bound to CIGB-552-B.

(a)

(b)