Proteomic Study to Survey the CIGB-552 Antitumor Effect

Table 3

Differentially expressed proteins in HT-29 cells treated with CIGB-552.

UniProt ACC	Description	Gene symbol	Score	Fold change
				40 min (2.6)	2 h (2.6)	5 h (2.4)
Downregulated proteins
Q14566	DNA replication licensing factor MCM6	MCM6	76.01	−4.2	−1.1	−2
Q14764	Major vault protein	MVP	88.9	−1.2	−1.9	−2.6
P35579	Myosin-9	MYH9	166.85	−1.7	−2.9	−1.6
P61160	Actin-related protein 2	ACTR2	60.97	−2.6	−1.4	−1.8
Q02338	D-beta-hydroxybutyrate dehydrogenase, mitochondrial	BDH1	71.17	−1.7	−1.4	−2.6
P20290	Transcription factor BTF3	BTF3	62.09	1.2	−2.9	−1.5
Q7L1Q6	Basic leucine zipper and W2 domain-containing protein 1	BZW1	95.07	−1.3	−3.8	−2.3
P17655	Calpain-2 catalytic subunit	CAPN2	101.09	−1.3	−3.1	−1.9
A8K010	Putative uncharacterized protein C6orf176	C6orf176	33.72	−1.1	−3.3	−1.6
P53618	Coatomer subunit beta	COPB1	64.4	−2.9	−1.5	−1.4
O60888	Protein CutA	CUTA	62.42	1.2	−2.2	−2.9
O14976	Cyclin-G-associated kinase	GAK	47.67	−3.4	−3.8	−2.9
O95395	Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3	GCNT3	59.62	−3.2	−1.2	−1.6
P52306	Rap1 GTPase-GDP dissociation stimulator 1	RAP1GDS1	66.74	−1.2	−3	−1.1
O15228	Dihydroxyacetone phosphate acyltransferase	GNPAT	46.58	−4.5	−3.1	−1.9
Q86Y56	HEAT repeat-containing protein 2	HEATR2	63.07	−1.5	−5	−1.4
Q6YP21	Kynurenine-oxoglutarate transaminase 3	CCBL2	39.9	1.7	OFF	1.5
P43243	Matrin-3	MATR3	269.16	1	−1.2	−4.5
O75439	Mitochondrial-processing peptidase subunit beta	PMPCB	99.73	−2.9	1.2	−1.1
O43847	Nardilysin	NRD1	41.16	−1.3	−2.9	1.6
O15270	Serine palmitoyltransferase 2	SPTLC2	62.71	−3.2	−2	−2.5
P29122	Proprotein convertase subtilisin/kexin type 6	PCSK6	30.52	−1.3	−1.5	−16.7
P00491	Purine nucleoside phosphorylase	PNP	91.96	−2	−5.6	1.7
P25787	Proteasome subunit alpha type 2	PSMA2	75.42	1.6	−2.9	1
O14818	Proteasome subunit alpha type 7	PSMA7	103.36	−1.3	−2.8	−1.1
Q13813	Spectrin alpha chain, nonerythrocytic 1	SPTAN1	112.76	−1.6	−3.1	−1.6
Q9UL25	Ras-related protein Rab-21	RAB21	129.33	−2.6	1.1	−1.1
Q9NV58	E3 ubiquitin-protein ligase RNF19A	RNF19A	31.17	−1.4	−1.8	−4.8
P62081	40S ribosomal protein S7	RPS7	65.3	−4.2	−3.8	−2.6
Q96FQ6	Protein S100-A16	S100A16	78	−2.6	−1.4	1
P63208	S-phase kinase-associated protein 1	SKP1	72.23	−7.1	−3.1	1.2
O00193	Small acidic protein	SMAP	74.25	−1.5	−2.6	−1.3
Q96JX3	Protein SERAC1	SERAC1	32.13	−1.1	1.3	−2.5
Q9Y5M8	Signal recognition particle receptor subunit beta	SRPRB	54.95	−1.7	−1.1	−2.9
Q96I99	Succinyl-CoA ligase [GDP-forming] subunit beta, mitochondrial	SUCLG2	85.7	−1.5	−2.4	−2.4
Upregulated proteins
O75223	Gamma-glutamylcyclotransferase	GGCT	37.33	1.1	2	3.8
P61758	Prefoldin subunit 3	VBP1	152.64	2.1	2.2	2.5
Q9BWD1	Acetyl-CoA acetyltransferase, cytosolic	ACAT2	69.2	2.5	2	3.2
Q86TX2	Acyl-coenzyme A thioesterase 1	ACOT1	73.32	2.8	1.6	2.4
P02647	Apolipoprotein A-I	APOA1	76.16	1.9	2.7	−1.1
O00192	Armadillo repeat protein deleted in velocardiofacial syndrome	ARVCF	46	ON	ON	ON
Q9NYF8	Bcl-2-associated transcription factor 1	BCLAF1	86.18	1	ON	ON
O14523	C2 domain-containing protein 2-like	C2CD2L	31.3	2.9	1.7	2
P60953	Cell division control protein 42 homolog	CDC42	52.58	2.3	1.5	3.1
Q9UJS0	Calcium-binding mitochondrial carrier protein Aralar2	SLC25A13	57.95	2.4	3.4	1.9
O43809	Cleavage and polyadenylation specificity factor subunit 5	NUDT21	70.35	1.2	−1.7	2.5
Q96C86	Scavenger mRNA-decapping enzyme DcpS	DCPS	70.59	1.5	2.1	2.4
Q5TDH0	Protein DDI1 homolog 2	DDI2	31.55	1	ON	ON
P18858	DNA ligase 1	LIG1	49.53	2.3	2.2	2.6
Q5JZY3	Ephrin type A receptor 10	EPHA10	37.22	ON	ON	ON
O76003	Glutaredoxin-3	GLRX3	50.31	3.2	1.8	2.4
Q14103	Heterogeneous nuclear ribonucleoprotein D0	HNRNPD	138.39	1.4	3	1.4
Q96P70	Importin-9	IPO9	88.04	3.9	2.9	2.9
P33176	Kinesin-1 heavy chain	KIF5B	102.25	4.3	3.6	5.1
P49006	MARCKS-related protein	MARCKSL1	112.06	ON	ON	1
Q9H3R2	Mucin-13	MUC13	68.36	1.8	4.2	1.9
Q7Z406	Myosin-14	MYH14	90.59	−1.1	9.7	−1.9
Q9NX40	OCIA domain-containing protein 1	OCIAD1	87.8	2.6	2.9	2.3
Q15102	Platelet-activating factor acetylhydrolase IB subunit gamma	PAFAH1B3	84.5	2.4	62.4	1.6
O43252	Bifunctional 3′-phosphoadenosine 5′-phosphosulfate synthetase 1	PAPSS1	51.9	1	1	ON
P36871	Phosphoglucomutase-1	PGM1	105.24	3.3	−2.2	2.2
P35232	Prohibitin	PHB	90.52	8.4	4.7	3.2
Q9UHJ6	Sedoheptulokinase	SHPK	59.09	1	1	ON
P52788	Spermine synthase	SMS	60.56	−1.2	2.1	3.2
Q01082	Spectrin beta chain, brain 1	SPTBN1	63.84	ON	ON	ON
Q9Y3F4	Serine-threonine kinase receptor-associated protein	STRAP	80.3	2.9	3.7	4.5
P26640	Valyl-tRNA synthetase	VARS	54.77	1.7	3	3.5
Q86VP1	Tax1-binding protein 1	TAX1BP1	31.29	2.3	11.7	1.2
Q9HD45	Transmembrane 9 superfamily member 3	TM9SF3	71.54	1.5	3.1	−1.8
P55327	Tumor protein D52	TPD52	64.69	5.9	1.8	1.8
P38606	V-type proton ATPase catalytic subunit A	ATP6V1A	66.31	−1.2	4.4	2.8
P45880	Voltage-dependent anion-selective channel protein 2	VDAC2	59.14	2.1	4	2

UniProtKB/Swiss-Prot entry accession number (http://www.uniprot.org/). The UniProt accession numbers highlighted in bold indicate those proteins (8) that were also identified by chemical proteomics.
Recommended gene name (official gene symbol) as provided by UniProtKB/Swiss-Prot.
MASCOT protein score.
Fold change of differentially regulated proteins after 40 min, 2 h, and 5 h of treatment with CIGB-552 [(−) downregulated]. For each incubation time with CIGB-552, is given the fold change threshold to consider a protein significantly up- or downregulated. The fold changes that have an absolute value higher than the threshold are highlighted in boldface.