Morphine Reduces Myocardial Infarct Size via Heat Shock Protein 90 in Rodents

Figure 2

Experimental protocol description. (a) Pictorial description of the rat in vivo myocardial infarction protocol. Surgical preparation involved a tracheotomy, carotid cannulation to measure blood pressure, and internal jugular vein cannulation to deliver drugs. Ischemia was generated by snaring the left anterior descending coronary artery and subsequently releasing the snare for reperfusion. (b) Experimental protocol. After 30 minutes of baseline, rats were subjected to treatment and subsequently 30 minutes of ischemia followed by 2 hours of reperfusion. HSP90 inhibitors were given 5 minutes prior to morphine, SB216763, or vehicle. (c) Representative staining for infarct size. After 2 hours of reperfusion, the LAD was again occluded and the area at risk was negatively stained by patent blue dye (far left). Following, the left ventricle was sliced into five equal pieces (middle). Lastly, the tissue was stained for viable tissue which turned red, while nonviable infarcted tissue remained white (far right). BL = baseline, RX = drug treatment, OCC = ischemia, and REP = reperfusion. Blue arrow = saline, orange arrow = morphine, green arrow = SB, and black arrow = RAD or DSG.

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