Immunohistological Analysis of ABCD3 Expression in Caucasian and African American Prostate Tumors

Figure 3

Detection of ABCD3 immunostaining in African American BPH and prostate tumors. A representative image of BPH from AA is shown in (a), while (b) and (c) are representative images of low Gleason tumors and high Gleason tumors, respectively. Figure 3(d) shows H&E staining in high Gleason tumor. ABCD3 staining was very strong in BPH and low and high Gleason tumors. Staining appeared in the peroxisomal membrane. We also observed staining in what appeared to be blebbing of peroxisomal membranes. ABCD3 staining pooled in the cytoplasm. Low Gleason tumors exhibited strong (≥3) staining in the peroxisomal membrane and strong (≥3) staining in glandular epithelial cells and in the stroma. Representative image of high Gleason tumor shown in (c) also exhibited strong ABCD3 staining (≥3) in blebbing peroxisomal membranes, with sporadic ABCD3 pooling in the cytoplasm and staining in the stroma. All images shown are at 600x magnification. A representative image of low Gleason tumor (Gleason 5-6; see Figure 3(b)) exhibited strong staining in the peroxisomal membrane and strong staining in glandular epithelial cells and in the stroma. High Gleason score tumors (Gleason 7-8; see Figure 3(c)) also exhibited strong ABCD3 staining (>3) with noticeable blebbing in the peroxisomal membranes, random ABCD3 pooling in the cytoplasm, and staining in the stroma. Scoring for ABCD3 staining intensities ranged from 0 to 3.

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