Magnolia officinalis Extract Contains Potent Inhibitors against PTP1B and Attenuates Hyperglycemia in db/db Mice

Figure 5

Effects of ME on GLUT4 translocation. Differentiated 3T3-L1 adipocytes (a) and C2C12 myotubes (b) were starved for 4 h before experiment. The cells were incubated with vehicle or ME at various concentrations for 30 min and then stimulated with vehicle or 10 nM insulin for 5 min. The cell membrane fractions were harvested. GLUT4 and GLUT1 were determined by Western blotting with anti-GLUT4 and anti-GLUT1 antibodies. Quantities of GLUT4 in cell membrane fractions were normalized against GLUT1 content and then calculated as fold changes of the insulin alone. Data are presented as mean ± SEM (). , , versus insulin alone.

(a)

(b)