Biotechnological Aspects and Perspective of Microbial Keratinase Production

Figure 4

Purification strategy for keratinases. Options with acidic, neutral, and alkaline keratinases are shown. Peak profiles indicate the individual proteins. Conventional purification strategies include ammonium sulphate precipitation followed by ion-exchange and gel-filtration. Other methods such as immunochromatography, high-performance liquid chromatography, and fast protein liquid chromatography are also involved in the purification of keratinases.