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BioMed Research International
Volume 2015 (2015), Article ID 145903, 9 pages
http://dx.doi.org/10.1155/2015/145903
Research Article

Improvement of Starch Digestion Using α-Amylase Entrapped in Pectin-Polyvinyl Alcohol Blend

1Departamento de Química, Instituto Federal de Educação Ciência e Tecnologia de Goiás, 76400-000 Uruaçu, GO, Brazil
2Departamento de Bioquímica e Biologia Molecular, Laboratório de Química de Polímeros, Universidade Federal de Goiás, Caixa Postal 131, 74001-970 Goiânia, GO, Brazil
3Departamento de Ciências Fisiológicas, Laboratório de Fisiologia da Digestão, Universidade Federal de Goiás, Caixa Postal 131, 74001-970 Goiânia, GO, Brazil
4Laboratório de Biotecnologia, Unidade Universitária de Ciências Exatas e Tecnológicas, Universidade Estadual de Goiás, 75.132-903 Anápolis, GO, Brazil

Received 22 December 2014; Revised 21 March 2015; Accepted 22 March 2015

Academic Editor: Zheng Guo

Copyright © 2015 Maurício Cruz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Polyvinyl alcohol (PVA) and pectin blends were used to entrap α-amylase (Termamyl) using glutaraldehyde as a cross-linker. The effect of glutaraldehyde concentration (0.25, 0.5, 0.75, 1.0, and 1.25%) on the activity of the immobilized enzyme and rate of enzyme released was tested during a 24 h period. Characteristics of the material, such as scanning electron microscopy (SEM), tensile strength (TS), elongation, and rate of dissolution in water (pH 5.7), ruminal buffering solution (pH 7.0), and reactor containing 0.1 mol L−1 sodium phosphate buffer (pH 6.5), were also analyzed. SEM results showed that the surfaces of the pectin/PVA/amylase films were highly irregular and rough. TS values increased as a function of glutaraldehyde concentration, whereas percentage of elongation (%E) decreased. Pectin/PVA/amylase films presented similar values of solubility in the tested solvents. The material obtained with 0.25% glutaraldehyde performed best with repeated use (active for 24 h), in a phosphate buffer reactor. By contrast, the material obtained with 1.25% glutaraldehyde presented higher performance during in vitro testing using an artificial rumen. The results suggest that pectin/PVA/amylase is a highly promising material for biotechnological applications.