β-Elemene Inhibits Cell Proliferation by Regulating the Expression and Activity of Topoisomerases I and IIα in Human Hepatocarcinoma HepG-2 Cells

Figure 5

The effects of β-ELE (β-Elemene) on TOPO I and TOPO IIα protein expression in HepG-2 cells. (a) Western blot analysis showed that both the ratios of TOPO I/β-actin (, , and ) and TOPO IIα/β-actin (, , and ) were significantly decreased in a dose-dependent manner after β-ELE treatment (0, 20, 40, and 60 μg/mL, ). (b) The ratios of TOPO I/β-actin (, , and ) were significantly lower than that of the control group (); and the ratios of TOPO IIα/β-actin (, , and ) were significantly lower than that of the control group () (, ). These results suggest that the expression of the TOPO I and TOPO IIα proteins significantly decreased in a dose-dependent manner after β-ELE treatment.

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