PI3K/AKT/mTOR/p70S6K Pathway Is Involved in Aβ25-35-Induced Autophagy

Figure 4

The effects of Aβ25-35 treatment on the LC3 II/I ratio and the levels of phosphorylation of AKT and p70S6K (S6K) in HT22 cells and C57 mice. (a) HT22 cells were treated with 40 μM Aβ25-35 for various times ranging from 0 h to 48 h. Rapamycin (1 μM, 48 h) served as a positive control. (b) The HT22 cells were divided into an untreated group, a 10 μM Aβ25-35 group, and a 40 μM Aβ25-35 group and treated for 24 h. The column graphs below the panels depict the relative expressions of LC3 II/I and phosphorylated AKT and p70S6K (S6K). (c) The mice were intracerebroventricularly administered sterile saline, low-dose Aβ25-35 (0.3 μL), or high-dose Aβ25-35 (0.6 μL). The expressions of Tublin confirmed equal protein loading. .

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