Potential of Newborn and Adult Stem Cells for the Production of Vascular Constructs Using the Living Tissue Sheet Approach

Figure 3

Expression of SMC differentiation markers in MSCs-derived vessels. Cross sections of tissue-engineered vessels made from cultured UCB-MSCs (a, e, i, and m), BMSCs (b, f, j, and n), SMCs (c, g, k, and o), or DFs (d, h, l, and p) were immunostained (red) for α-SMA (a–d), calponin (e–h), h-caldesmon (i–l), and keratin-18 (m–p). Nuclei were counterstained with Hoechst (blue); scale bar: 100 μm.

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