Evolutionary and Expression Analysis of miR-#-5p and miR-#-3p at the miRNAs/isomiRs Levels

Table 1

Frequency of nucleotide compositions between all human miRNAs from different arms.

Nucleotides	5p-miRNA (%)	3p-miRNA (%)	miR-#-5p (%)	miR-#-3p (%)	,
AA	18.38	18.13	17.98^c	17.09
UU	22.21	24.39	22.59	25.68
CC	17.39	30.64	17.80	36.06
GG	42.02	26.84	41.63	21.17
Total	100	100	100	100	21.31, 0.01
AAA	16.26^a	17.77	14.39^d	16.21
UUU	20.83	23.41	21.36	24.98
CCC	15.38	33.80	15.40	40.90
GGG	47.53	25.02	48.85	17.91
Total	100	100	100	100	42.74, 0.00
AAAA	17.72^b	19.87	14.97^e	17.95
UUUU	21.44	22.03	20.38	22.76
CCCC	8.97	34.99	8.60	45.83
GGGG	51.86	23.11	56.05	13.46
Total	100	100	100	100	81.10, 0.00

The percentage is estimated based on frequency in all the 5p- or 3p-miRNAs, all the miR-#-5p or miR-#-3p. ^aA significant difference in the triple repetitive nucleotides can be detected between 5p-miRNA and 3p-miRNA ( = 14.82, ), ^ba significant difference in the four repetitive nucleotides can be detected between 5p-miRNA and 3p-miRNA ( = 26.71, ), ^ca significant difference in the double repetitive nucleotides can be detected between miR-#-5p and miR-#-3p ( = 13.21, ), ^da significant difference in the triple repetitive nucleotides can be detected between miR-#-5p and miR-#-3p ( = 26.89, ), and ^ea significant difference in the four repetitive nucleotides can be detected between miR-#-5p and miR-#-3p ( = 52.17, ).