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BioMed Research International
Volume 2015 (2015), Article ID 172756, 8 pages
Research Article

Immunohistochemical Expression of Ornithine Decarboxylase, Diamine Oxidase, Putrescine, and Spermine in Normal Canine Enterocolic Mucosa, in Chronic Colitis, and in Colorectal Cancer

1School of Biosciences and Veterinary Medicine, University of Camerino, Via Circonvallazione 93/95, 62024 Matelica, Italy
2S. Antonio Clinic, 26020 Madignano, Italy
3Faculty of Veterinary Medicine, 050097 Bucharest, Romania
4Gastroenterology and Hepatology Section, Department of Medicine, Santa Maria della Misericordia Hospital, Piazzale Menghini 1, San Sisto, 06153 Perugia, Italy

Received 29 May 2015; Revised 3 August 2015; Accepted 10 September 2015

Academic Editor: Atsushi Sakuraba

Copyright © 2015 Giacomo Rossi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


We compared the immunohistochemical expression of putrescine (PUT), spermine (SPM), ornithine decarboxylase (ODC), and diamine oxidase (DAO) in bioptic samples of canine colonic mucosa with chronic inflammation (i.e., granulomatous colitis and lymphoplasmacytic colitis) or neoplasia. Single and total polyamines levels were significantly higher in neoplastic tissue than in normal samples. Samples with different degrees of inflammation showed a general decrease expression of ODC if compared to controls; SPM was practically not expressed in control samples and very low in samples with chronic-granulomatous inflammation. In carcinomatous samples, the ODC activity was higher with respect to controls and samples with inflammation. This is the first description of polyamines expression in dog colonic mucosa in normal and in different pathological conditions, suggesting that the balance between polyamine degradation and biosynthesis is evidently disengaged during neoplasia.