Table of Contents Author Guidelines Submit a Manuscript
BioMed Research International
Volume 2015, Article ID 175291, 8 pages
http://dx.doi.org/10.1155/2015/175291
Research Article

Ox-LDL Induces Dysfunction of Endothelial Progenitor Cells via Activation of NF-B

1Department of Cardiology, the Second Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China
2Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350112, China

Received 21 August 2014; Accepted 25 September 2014

Academic Editor: Ji Li

Copyright © 2015 Kang-ting Ji et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Dyslipidemia increases the risks for atherosclerosis in part by impairing endothelial integrity. Endothelial progenitor cells (EPCs) are thought to contribute to endothelial recovery after arterial injury. Oxidized low-density lipoprotein (ox-LDL) can induce EPC dysfunction, but the underlying mechanism is not well understood. Human EPCs were cultured in endothelial growth medium supplemented with VEGF (10 ng/mL) and bFGF (10 ng/mL). The cells were treated with ox-LDL (50 µg/mL). EPC proliferation was assayed by using CCK8 kits. Expression and translocation of nuclear factor-kabba B (NF-κB) were evaluated. The level of reactive oxygen species (ROS) in cells was measured using H2DCF-DA as a fluorescence probe. The activity of NADPH oxidase activity was determined by colorimetric assay. Ox-LDL significantly decreased the proliferation, migration, and adhesion capacity of EPCs, while significantly increased ROS production and NADPH oxidase expression. Ox-LDL induced NF-κB P65 mRNA expression and translocation in EPCs. Thus ox-LDL can induce EPC dysfunction at least by increasing expression and translocation of NF-κB P65 and NADPH oxidase activity, which represents a new mechanism of lipidemia-induced vascular injury.