BioMed Research International / 2015 / Article / Fig 2

Research Article

Evaluation and Application of the Strand-Specific Protocol for Next-Generation Sequencing

Figure 2

The expression correlation of transcripts between SS protocol and NSS protocol. The RPKM values of nonoverlapped transcripts were transformed with logarithm function with base 2. To avoid extreme values, only the transcripts with read count larger than 0.0001 in both libraries were considered. (a) The scatter plot of RPKM values derived from the SS and NSS libraries. The expression profile of nonoverlapped transcripts derived by SS protocol is highly correlated with the one by NSS protocol. (b) Based on the overlap percentage, the overlapped transcripts were classified into sets and the correlation coefficients were calculated in the sets (real). According to the sizes of different overlap sets (Supplementary Table  2), we resampled the nonoverlapped transcripts to mimic the correlation coefficient of overlapped sets. By repeating 100 times, the average values of correlation coefficient were drawn (mimic).

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