BioMed Research International / 2015 / Article / Fig 3

Research Article

Evaluation and Application of the Strand-Specific Protocol for Next-Generation Sequencing

Figure 3

The real-time PCR evaluation of SS and NSS protocol. We used real-time PCR as standard to examine which protocol is less biased in determining expression profile of overlapped transcripts. After deriving the fold changes from Ct, SS RPKM and NSS RPKM, 81 values of fold change were collected. For a less biased result, we used bootstrap resampling for 100 times.

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