BioMed Research International / 2015 / Article / Fig 4

Research Article

Evaluation and Application of the Strand-Specific Protocol for Next-Generation Sequencing

Figure 4

The evaluation of the performance and strand specificity. (a) The comparison of coverage between SS and NSS protocol. The lengths of all transcripts were normalized to 100 bases. Y axis denotes coverage frequency normalized with the number of all mapped bases. (b) Y axis denotes the mappable rate. For all libraries, including the SS library, the reads located at the sense and antisense strands are summarized. (c) Y axis denotes the sense strand mappable rate divided by the antisense strand one. (d) Y axis denotes the logarithm (base 2) values of ratios of the sense strand read count to the antisense strand one. at Y axis denotes −2.9357, indicating that 13.07% of the mappable reads from the SS library are located at the antisense strand of the transcripts. To eliminate dramatic values owing to ultimately small read count, only the nonoverlapped transcripts with read count ≥10 (18,573) at both strands were analyzed.

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