BioMed Research International / 2015 / Article / Fig 5

Research Article

Evaluation and Application of the Strand-Specific Protocol for Next-Generation Sequencing

Figure 5

Illustration of novel antisense transcripts. (a) The thick and thin bars denote exon and intron, respectively. The terminal arrows denote the orientations of genes in chromosome. Right-ward arrow and left-ward arrow denote plus and minus strand, respectively. The blue and green bars denote known transcript and novel-antisense transcripts. The black thin arrows denote the positions of PCR primers. (b) NAG0001-1 and NAG0001-2 were detected with the same primer set. NAG0002-1 and NAG0002-2 were detected with the same primer set. We designed the PCR primers to avoid amplifying the antisense known transcripts. (c) The cloning and sequencing confirm the PCR results. The blue arrows mark the exon-exon junctions.

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