BioMed Research International / 2015 / Article / Fig 5

Research Article

Evaluation and Application of the Strand-Specific Protocol for Next-Generation Sequencing

Figure 5

Illustration of novel antisense transcripts. (a) The thick and thin bars denote exon and intron, respectively. The terminal arrows denote the orientations of genes in chromosome. Right-ward arrow and left-ward arrow denote plus and minus strand, respectively. The blue and green bars denote known transcript and novel-antisense transcripts. The black thin arrows denote the positions of PCR primers. (b) NAG0001-1 and NAG0001-2 were detected with the same primer set. NAG0002-1 and NAG0002-2 were detected with the same primer set. We designed the PCR primers to avoid amplifying the antisense known transcripts. (c) The cloning and sequencing confirm the PCR results. The blue arrows mark the exon-exon junctions.
(a)
(b)
(c)

We are committed to sharing findings related to COVID-19 as quickly and safely as possible. Any author submitting a COVID-19 paper should notify us at help@hindawi.com to ensure their research is fast-tracked and made available on a preprint server as soon as possible. We will be providing unlimited waivers of publication charges for accepted articles related to COVID-19. Sign up here as a reviewer to help fast-track new submissions.