BioMed Research International / 2015 / Article / Tab 1

Research Article

Evaluation and Application of the Strand-Specific Protocol for Next-Generation Sequencing

Table 1

The qPCR and RPKM results of the examined genes. We used qPCR to examine the expressions of nine overlapped genes and three nonoverlapped genes. EEF1A1 and HPRT1 are two commonly used internal control genes, while TMEM66 is the new internal control gene in this study. RPKM_SS and RPKM_NSS denote the RPKM values determined by the SS and NSS protocols, respectively. Ct1, Ct2, and Ct3 denote the Ct values from three independent qPCR experiments.

GeneRPKM_SSRPKM_NSSCt1Ct2Ct3

AMDHD23.585448.1644126.3030126.5186426.41083
ATP6V1C22.3121932.66132.1800531.5789531.8795
COX1123.4910432.5610221.2781921.2783721.27828
DALRD35.2400317.9718423.2654723.2496923.25758
MXD31.5098378.5801436.0633335.2924735.6779
POLR2I8.0170625.970719.3986819.4280619.41337
SDR39U16.9697120.545323.0252623.0547423.04
SYNC1.3060665.6976928.1473828.0556728.10153
TMOD11.300334.3222627.9830727.8480527.91556
EEF1A12248.532270.1113.1113913.1532113.13354
HPRT128.015128.325420.8835821.0769620.98027
TMEM66126.355126.43718.8552418.8588218.85703

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