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BioMed Research International
Volume 2015, Article ID 185841, 7 pages
Research Article

Alginate/Poly(γ-glutamic Acid) Base Biocompatible Gel for Bone Tissue Engineering

1Department of Radiology, Wan Fang Hospital, Taipei Medical University, Taipei 110, Taiwan
2Department of Radiology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan
3Department of Health Healing and Health Marketing, Kainan University, Taoyuan 338, Taiwan
4Department of Mechanical Engineering, National Taiwan University of Science and Technology, Taipei 106, Taiwan
5Department of Materials Science and Engineering, National Taiwan University of Science and Technology, Taipei 106, Taiwan
6Graduate Institute of Clinical Medicine and Graduate Institute of Medical Sciences and Graduate Institute of Biomaterials, Taipei Medical University, Taipei 110, Taiwan

Received 29 April 2015; Revised 5 August 2015; Accepted 6 August 2015

Academic Editor: Iulian Antoniac

Copyright © 2015 Wing P. Chan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A technique for synthesizing biocompatible hydrogels by cross-linking calcium-form poly(γ-glutamic acid), alginate sodium, and Pluronic F-127 was created, in which alginate can be cross-linked by Ca2+ from Ca–γ-PGA directly and γ-PGA molecules introduced into the alginate matrix to provide pH sensitivity and hemostasis. Mechanical properties, swelling behavior, and blood compatibility were investigated for each hydrogel compared with alginate and for γ-PGA hydrogel with the sodium form only. Adding F-127 improves mechanical properties efficiently and influences the temperature-sensitive swelling of the hydrogels but also has a minor effect on pH-sensitive swelling and promotes anticoagulation. MG-63 cells were used to test biocompatibility. Gelation occurred gradually through change in the elastic modulus as the release of calcium ions increased over time and caused ionic cross-linking, which promotes the elasticity of gel. In addition, the growth of MG-63 cells in the gel reflected nontoxicity. These results showed that this biocompatible scaffold has potential for application in bone materials.