Identification, Characterization, and Developmental Expression Pattern of Type III Interferon Receptor Gene in the Chinese Goose

Table 1

The list of primers.

Primer name	Sequence 5′-3′	Application
Oligo(dT)18	TTTTTTTTTTTTTTTTTT	Reverse transcription
IFNLR1-1F	ACATGG(A/G)C(T/G)CC(A/G)GGAGAAGG	Degenerate PCR
IFNLR1-1R	TCTTGTCTTCAGA(T/C)CC(G/A/T/C)GC	Degenerate PCR
IFNLR1-5G0	AGGCGGCATTCAC	5RACE PCR
IFNLR1-5GSP1	AGCCAGTTCCACATCCAAAT	5RACE PCR
IFNLR1-5GSP2	TCCTGGCTTTCATACCTCAC	5RACE PCR
5RACE-AAP	GGCCACGCGTCGACTACGGGIIGGGIIGG; GIIGGGIIG	5RACE PCR
5RACE-AUAP	GGCCACGCGTCGACTAGTAC	5RACE PCR
IFNLR1-3GSP1	CTATTTGGATGTGGAACTGGCTC	3RACE PCR
IFNLR1-3GSP2	CCTGGATGTATGACTTGAACC	3RACE PCR
3RACE-AP	GGCCACGCGTCGACTAGTACT(17)	3RACE PCR
3RACE-AUAP	GGCCACGCGTCGACTAGTAC	3RACE PCR
IFNLR1-2F	ATGCTCCTCCATAAGTCG	Full-length amplification
IFNLR1-2R	GCTGTGACTTCTCCCTGA	Full-length amplification
IFNLR1-3F	TCTGAAGACAAGAAGCAAT	qRT-PCR
IFNLR1-3R	GCACACTGGTTGGGAGAAT	qRT-PCR
β-actin-F	CCGTGACATCAAGGAGAA	qRT-PCR
β-actin-R	GAAGGATGGCTGGAAGAG	qRT-PCR