The Role and Mechanism of α-Klotho in the Calcification of Rat Aortic Vascular Smooth Muscle Cells

Figure 3

α-SMA was downregulated during the calcification of VSMCs. VSMCs were treated with or without 10 mM β-GP and with or without 50 ng/mL Klotho for 12 d. (a) Representative α-SMA expression levels from VSMCs following treatment with control medium, β-GP medium, or β-GP + 50 ng/mL rmKlotho medium for 12 days. (b) The quantification of α-SMA is represented as the mean with the SE ( per group) for each group in its respective column shown in (b). versus control group; versus β-GP group. Scale bar = 100 μm.

(a)

(b)