Research Article

Cytotoxicity of Cyclodipeptides from Pseudomonas aeruginosa PAO1 Leads to Apoptosis in Human Cancer Cell Lines

Figure 5

Apoptotic morphological changes in HeLa and Caco-2 cells induced by cyclodipeptides from Pseudomonas aeruginosa. Images of cells were taken under phase-contrast inverted microscopy after treatment with the P. aeruginosa CDP mix for 24 h. HeLa cell treatment: (a) DMSO (0.05%; negative control); (b) actinomycin D (50 mg/mL; positive apoptosis control); ((c)–(f)) CDP mix at a concentration of 0.1, 1.0, 10, and 100 mg/mL, respectively. Caco-2 cell treatment: (g) DMSO (0.05%; negative control); (h) actinomycin D (50 mg/mL; positive apoptosis control); ((i)–(l)) CDP mix at a concentration of 0.1, 1.0, 10, and 100 mg/mL, respectively.
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