BioMed Research International / 2015 / Article / Fig 1

Research Article

Intratracheal Bleomycin Aerosolization: The Best Route of Administration for a Scalable and Homogeneous Pulmonary Fibrosis Rat Model?

Figure 1

Evolution of histological alterations in rat lungs after BLM instillation. (a) Trichrome Blue staining in sham animals and in treated rats, 3, 7, 14, 21, and 56 days (3 d, 7 d, 14 d, 21 d, and 56 d) after bleomycin (BLM) instillation. Collagen is stained in blue and cells in red. Magnification: 100x. (b) Average number of cells per mm² lung surface in sham animals (in white) and 3, 7, 14, 21, and 56 days after BLM instillation (in grey). (c) Average percentage (%) of lung area occupied by collagen in sham animals (in white) and 3, 7, 14, 21, and 56 days after BLM instillation (in grey). (d) Quantification of lung fibrosis using a modified Ashcroft score in sham animals (in white) and 3, 7, 14, 21, and 56 days after BLM instillation (in grey; per time-point). (b) Sham versus every other time-points; (14 + 21 d) versus (3, 7, and 56 d); ANOVA one way followed by Duncan’s test. (c-d) early (3–7 d) versus later (14 to 56 d) time-points; ANOVA one way followed by a Duncan’s test.
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