Intratracheal Bleomycin Aerosolization: The Best Route of Administration for a Scalable and Homogeneous Pulmonary Fibrosis Rat Model?
Evolution of pulmonary histopathological alterations after BLM aerosolization. (a) Trichrome Blue staining in sham animals and 3, 7, 14, 21, and 56 days (3 d, 7 d, 14 d, 21 d, and 56 d) after bleomycin (BLM) aerosolization. Collagen is stained in blue and cells in red. Magnification: 100x. (b) Left panel: peribronchial lesions are present at late time-points (days 14, 21, and 56) as after BLM instillation. Right panel: destructive lesions are observed at days 14 and 56. (c) Average number of cells per mm² lung surface in sham animals (in white) and 3, 7, 14, 21, and 56 days after BLM aerosolization (in grey). (d) Quantification of lung fibrosis using a modified Ashcroft score in sham animals (in white) and 3, 7, 14, 21, and 56 days after BLM aerosolization (in grey; per time-point). (c) Sham versus every other time-points; : 14 d versus 3 d and 21 d; ANOVA one way followed by Duncan’s test. (d) early (3–7 d) versus late (14 to 56 d) time-points; ANOVA one way followed by a Duncan’s test.
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