Intratracheal Bleomycin Aerosolization: The Best Route of Administration for a Scalable and Homogeneous Pulmonary Fibrosis Rat Model?
Comparison of fibrosis evolution after BLM instillation or aerosolization. Fibrosis was quantified in each lung lobe using a modified Ashcroft score, in control (CTL, in black), sham (in white), and BLM animals, at 3, 7, 14, 21, and 56 days after BLM instillation (Instil., in dark grey) or aerosolization (Aerosol., in grey). Two different methods were applied for quantification, as described in Section 2: RS- (random sampling-) method (a) and MA- (most affected field-) method (b). For statistical analysis, data from CTL and sham animals were grouped (CTL + sham) as well as results concerning BLM rats at early (3–7 d) and late (14 to 56 d) time-points. Grouped means are not different as compared by a Mann Whitney rank sum tests (CTL versus Sham) or using Kruskal Wallis one way analysis (3 versus 7 d; 14 versus 21 versus 56 d). Groups were compared as indicated, using a Kruskal Wallis one way (pairwise multiple comparison of means, Dunn’s method). CTL: ; Sham: ; BLM Instil.: ; BLM Aerosol.: . : late versus early versus CTL + sham; : Aerosol. versus Instil. versus CTL + sham at late time-points. Kappa index representing interobserver agreement was for the instillation model of 0.12 and 0.09 (RS-method and the MA-method, resp.) and for the aerosolization model 0.64 and 0.43.
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