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Figure 3: Effects of decorin on growth and proliferation of trophoblast cells. ((a) and (b)) The proliferation ability of HTR-8/SVneo and JEG-3 cells was inhibited in pEGFP-DCN group compared to control, as identified by MTT assays. ((c) and (d)) Colony-forming assay showed a decrease of cells proliferation in pEGFP-DCN group compared to empty vector both in HTR-8/SVneo and in JEG-3 cells. ((e) and (f)) Cell-cycle analysis was performed 48 h following the treatment of HTR-8/SVneo and JEG-3 cells with pEGFP-DCN or empty vector. The DNA content was quantified by flow cytometric analysis. Values are represented as mean ± SEM ().