A Novel Matrine Derivative WM130 Inhibits Activation of Hepatic Stellate Cells and Attenuates Dimethylnitrosamine-Induced Liver Fibrosis in Rats

Figure 5

WM130 inhibits the activation of HSC-T6 cells and the secretion of ECM. (a) All samples were collected after incubation with WM130 and M19 for 24 h. Total RNA was extracted and the mRNA expression levels of α-SMA, Collagen I, and Collagen III in different groups were examined by RT-PCR assay. (b) Cells were lysed in RIPA lysis buffer supplemented with PMSF and the protein expression levels of α-SMA and MMP-2 in different groups were quantified by Western Blot assay. (c) Cell culture media in different groups were collected in which the concentrations of collagen I were measured by ELISA assay. All experiments were independently performed 3 times. , versus control group; ^#, ^## versus M19 68 μM group.

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