Inhibitory Effect of a Novel Antirheumatic Drug T-614 on the IL-6-Induced RANKL/OPG, IL-17, and MMP-3 Expression in Synovial Fibroblasts from Rheumatoid Arthritis Patients
The effect of T-614 on p-ERK1/2, ERK1/2 expression in IL-6-induced RASFs. (a) RASFs were stimulated with IL-6 for 0, 1, 6, 12, 24, and 48 h; total proteins were extracted and expression levels of ERK1/2 and p-ERK1/2 protein were determined by western blot. (b, c) RASFs were cultured with PD98059 (25 μM) for 1 h or not; then IL-6/sIL-6R were added, and incubation was continued for another 1 h. Total proteins were extracted and expression levels of ERK1/2 and p-ERK1/2 protein were determined by western blot. The data shown are the mean ± SD for three independent experiments, each in triplicate. ∗∗ and ∗.
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