Effect of 50 Hz Extremely Low-Frequency Electromagnetic Fields on the DNA Methylation and DNA Methyltransferases in Mouse Spermatocyte-Derived Cell Line GC-2

Figure 2

Effect of ELF-EMF electromagnetic field exposures on the mRNA and protein of DNMT1, DNMT3a, and DNMT3b in GC-2 cells. (a) The expression of DNMT1 was significantly lower than the sham-exposure group at magnetic field intensity of 1 mT and 2 mT and was significantly higher than the sham-exposure group at 3 mT. (b) The expression of DNMT3a decreased exposure to 50 Hz ELF-EMF exposure compared with the sham-exposure group at magnetic field intensity of 2 mT. (c) DNMT3b expression was significantly lower than the sham-exposure group at magnetic intensity of 1 mT and 2 mT and significantly higher than the sham-exposure group at 3 mT. (d) The protein expression of DNMT1 decreased at magnetic field intensity of 1 mT and increased at 3 mT.

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