A Heparan Sulfate-Binding Cell Penetrating Peptide for Tumor Targeting and Migration Inhibition

Figure 4

Inhibitory effect of CPPecp on angiogenesis in Tg(kdr:EGFP) zebrafish. (a) Morphology and green-labeled vessels in Tg(kdr:EGFP) zebrafish. The red rectangle represents the area of subintestinal vessel (SIV) network. Magnification: 100x. Scale bar: 400 μm. (b) Development of SIV network in the zebrafish yolk sac could be classified into three groups: normal, mild inhibition, and severe inhibition pattern. Magnification: 400x. Scale bar: 100 μm. (c) Development of SIV network in the zebrafish yolk sac at 24 h postinjection (hpi). Magnification: 400x. Scale bar: 100 μm. (d) Percentage of different SIV phenotypes in the zebrafish yolk sac at 24 hpi. (e) Percentage of severe inhibited SIV phenotype in ectopic SIV phenotype in the zebrafish yolk sac at 24 hpi. 16–20 zebrafish were used for each treatment group. The data represents means ± SD (standard deviation) of three independent experiments.

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