Use of Dried Plasma Spots for HIV-1 Viral Load Determination and Drug Resistance Genotyping in Mexican Patients

Table 2

Drug resistance mutations in the protease (PR) and reverse-transcriptase (RT) regions of HIV-1 identified in plasma and DPS paired samples.

Sample ID	HIV-1	Codons		Major drug resistance mutations
	Subtype	PR	RT
HIV-03-P	B	21–99	1–259	RT (RTNI): K219Q
HIV-03-DPS		22–99	1–249	RT (RTNI): K219Q
HIV-04-P	B	21–99	1–259	None
HIV-04-DPS		3–99	1–259	None
HIV-47-P	B	9–99	1–251	None
HIV-47-DPS		15–99	1–248	None
HIV-43-P	B	6–99	1–254	RT (RTNNI): V179D
HIV-43-DPS		36–99	1–230	RT (RTNNI): V179D
HIV-08-P	B	4–99	1–254	RT (RTNNI): V108I
HIV-08-DPS		3–99	1–249	RT (RTNNI): V108I
HIV-86-P	B	3–99	1–254	None
HIV-86-DPS		15–99	1–249	None
HIV-31-P	B	3–99	1–259	None
HIV-31-DPS		42–99	1–239	None
HIV-20-P	B	22–99	1–250	PR (PI): V82A
HIV-20-DPS		16–99	1–239	PR (PI): V82A, I50M
HIV-13-P	B	38–99	1–254	PR (PI): M46L, V82A, K43T, A71T, and G73CRT (RTNI): M41L, D67N, L74V, M184V, L210W, and K219Q; RT (RTNNI): Y188L
HIV-13-DPS		4–99	1–259	PR (PI): M46L, I54V^1, V82A, K43T, A71T, and G73CRT (RTNI): M41L, D67N, L74V, M184V, L210W, T215Y, and K219Q; RT (RTNNI): Y188L
HIV-22-P	B	1–99	1–332	PR (PI): N88S, L10F, and M46V; RT (RTNI): M41L, M184V, L210W, and T215Y
HIV-22-DPS		16–99	1–254	PR (PI): N88S, M46V; RT (RTNI): M41L, M184V, L210W, and T215Y

RTNNI: nonnucleoside reverse-transcriptase inhibitors; RTNI: nucleoside reverse-transcriptase inhibitors; and PI: protease inhibitors.
Major drug resistance mutations to PI.
Minors drug resistance mutations to PI.
P = liquid plasma sample.
DPS = dried plasma spot sample.
Not present in DPS paired sample; not present in plasma paired sample.