The Benefit of a Human Bone Marrow Stem Cells Concentrate in addition to an Inorganic Scaffold for Bone Regeneration: An In Vitro Study

Figure 6

Representative SEM images of GRHC pellets colonized with BM concentrate or BM aspirate, at 17 and 23 days. Pellets colonized with BM concentrate at 17 ((a) and (b)) and 23 ((c)–(e)) days; (a) and (c) clusters of cells on the material surface; (b) and (d) high magnification images showing the cells taking advantage of the microporosity to attach to the material surface (arrows); (e) formation of globular mineral deposits closely associated with the cell layer (arrows); (f) X-ray spectrum of the mineral deposits showing Ca and P peaks. (a) and (c) Bar, 100 μm; (b), (d), and (e) Bar, 50 μm. Pellets colonized with BM aspirate at 17 (a) and 23 ((b) and (c)) days; (a) and (b) clusters of cells (arrows) on the material surface; (c) high magnification image showing the cells attached to the microporous of the material surface (arrow). (a) and (b) Bar, 100 μm; (c) Bar, 50 μm.