Diverse Reductive Dehalogenases Are Associated with Clostridiales-Enriched Microcosms Dechlorinating 1,2-Dichloroethane

Figure 4

Neighbour-joining tree with branch length to assess the relationship between DcaA of the new RDs identified in the lower aquifer (LO-RD-X) and those previously characterized from the upper aquifer (RD-54 [16] and UP-RD-X [9]) and from D. dichloroeliminans strain DCA1 (RD-DCA1 [16]). Other A subunits of PceA of Dehalobacter restrictus strain DSMZ 9455T (RD-TCE D. restrictus: AJ439607), Desulfitobacterium hafniense strain TCE1 (RD-TCE D. hafniense: AJ439608), Desulfitobacterium sp. strain Y51 (RD-PCE D. Y51: AY706985), WL rdhA1 (FJ010189), and WL rdhA2 (FJ010190) are also reported. The numbers at each branch point represent percentage of bootstrap calculated from 1000 replicate trees. The scale bar represents the sequence divergence.