Application of FTIR-ATR Spectroscopy to Determine the Extent of Lipid Peroxidation in Plasma during Haemodialysis

Table 1

Assignment of organic compounds to bands on the FTIR-ATR spectrum of plasma lipids; -stretching vibration, -bending vibrations, s-symmetric, as-asymmetric, and sh-shoulder band.

Wave number/cm^−1		Assignment	Literature
On recorded spectra	According to literature
3370	3280–3473	(O–H) and (N–H): water molecules, choline	[24, 25]
2958 sh	2952–2959 sh	(CH3): lipids, cholesterol esters, fatty acids	[25–32]
2920	2919–2925	(CH2): lipids, long-chain fatty acids	[25–32]
2868 sh	2871–2873 sh	(CH3): lipids, fatty acids	[24, 25, 27]
2852	2850–2855	(CH2): lipids, long-chain fatty acids	[25–32]
1738	1732–1747	(C=O): lipids, cholesterol esters, fatty acids oxides	[25, 27–30, 32]
1712	1712–1718	(C=O): COOH group of fatty acids	[26, 32]
1495 sh	1490 sh	(N–CH3): choline group	[33]
1465	1464–1468	(CH2): aliphatic chains of fatty acids	[24–29]
1377	1377–1381	(CH3): aliphatic chains of fatty acids	[26–29]
1184	1160–1179	(C–O): lipid ester bonds	[27, 28]
1082	1075–1090	(PO2): phospholipids	[26, 27]
970	967–972	(C=C): conformation trans	[26, 28, 29]