Codon Optimization Significantly Improves the Expression Level of α-Amylase Gene from Bacillus licheniformis in Pichia pastoris

Figure 4

SDS-PAGE analysis of recombinant BlAmy. (a) SDS-PAGE of recombinant BlAmy in fermentation broth from 5- and 50-L bioreactor after methanol induced for 168 h. M: protein MW markers; lane 1 and lane 2: recombinant BlAmy and BlAmy-opt from 5-bioreactor, respectively. Lane 3 and lane 4: recombinant BlAmy and BlAmy-opt from 50-bioreactor, respectively. (b) Analysis of purified recombinant BlAmy and -deglycosylated recombinant BlAmy by Endo H. Lane 1: purified recombinant BlAmy; lane 2, lane 3, and lane 4: the -deglycosylated recombinant BlAmy and Endo H; lane 5: Endo H.

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