Acupuncture May Exert Its Therapeutic Effect through MicroRNA-339/Sirt2/NFB/FOXO1 Axis

Figure 3

Overexpression of miRNA-339 also inhibited Sirt2 expression in rat neurons. (a) Overexpression of miR-339 inhibited Sirt2 expression in a concentration-dependent manner. NGF-induced differentiated PC12 cells were treated with miRNA-339 mimic at 10 and 50 nM. Forty-eight hours later, the cell lysates were collected for western blotting. , versus control. (b) Overexpression of miR-339 inhibited Sirt2 expression in a time-dependent manner. NGF-differentiated PC12 cells were treated with miRNA-339 mimic at 50 nM for 24 and 48 h. At each time point, the cell lysates were collected for western blotting. , versus control.

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