BioMed Research International / 2015 / Article / Fig 4

Research Article

Acupuncture May Exert Its Therapeutic Effect through MicroRNA-339/Sirt2/NFB/FOXO1 Axis

Figure 4

Knockdown of miRNA-339 increased Sirt2 expression in rat neurons. (a) Knockdown of miR-339 increased Sirt2 expression in a concentration-dependent manner. NGF-induced differentiated PC12 cells were treated with miRNA-339 inhibitor at 10 and 50 nM. Forty-eight hours later, the cell lysates were collected for western blotting. , versus control. (b) Knockdown of miR-339 increased Sirt2 expression in a time-dependent manner. NGF-differentiated PC12 cells were treated with miRNA-339 inhibitor at 50 nM for 24 and 48 h. At each time point, the cell lysates were collected for western blotting. , versus control.
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