Research Article

Roles of ER and GPR30 in Proliferative Response of Human Bladder Cancer Cell to Estrogen

Figure 3

E2 induced activation of ERK through ERβ/GPR30-regulated EGFR-MAPK pathway in T24 cells. (a) E2 rapidly induced activation of ERK in T24 cells. Cells were transfected with specific siRNA against ERβ or GPR30, or pretreated with 100 nM AG1478 or 20 μM PD98059 for 30 min. Then 10 nM E2 was added and phosphorylated and total ERK levels were measured by western blot analysis. (b) Histogram of phosphorylation of ERK. The values were normalized to total ERK for each sample. The control was defined as 1.0. Blots are representative of three independent experiments with similar results. .
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