Differentiation between Acute Skin Rejection in Allotransplantation and T-Cell Mediated Skin Inflammation Based on Gene Expression Analysis
Table 1
Study groups and design.
Group and number of animals
Surgery procedure
Biopsy time point (day after transplantation)
Skin type
Biopsy region
Sample types provided
1 (ATC) = 5 (thigh) = 5 (footpad)
Orthotopic hind limb transplantation
Postoperative day (POD) 5
Hairy skin
Thigh
Allografts without immunosuppression
Nonhairy skin
Footpad
Allografts without immunosuppression
2 (ISO) = 5 (thigh) = 5 (footpad)
Orthotopic hind limb transplantation
Postoperative day (POD) 5
Hairy skin
Thigh
Isografts
Nonhairy skin
Footpad
Isografts
3 (CHS) = 5
CHS reaction on ear
24 hours after challenge
Hairy skin
Pinna
Skin inflammation
4 (CHS-CTRL) = 5
CHS reaction on ear
24 hours after challenge
Hairy skin
Pinna
Skin inflammation (control)
5 (DTH) = 5
DTH reaction on footpad
24 hours after challenge
Nonhairy skin
Footpad
Skin inflammation
6 (DTH-CTRL) = 5
DTH reaction on footpad
24 hours after challenge
Nonhairy skin
Footpad
Skin inflammation (control)
ATC: rejection group (donor: brown Norway (BN) rat, recipient: Lewis (LEW) rat, no immunosuppression). ISO: isograft group (donor: LEW rat, recipient: LEW rat, syngeneic control group). CHS: contact hypersensitivity group. CHS-CTRL: contact hypersensitivity control group. DTH: delayed type hypersensitivity group. DTH-CTRL: delayed type hypersensitivity control group.
