Thymoquinone-Loaded Nanostructured Lipid Carrier Exhibited Cytotoxicity towards Breast Cancer Cell Lines (MDA-MB-231 and MCF-7) and Cervical Cancer Cell Lines (HeLa and SiHa)

Table 3

Cytotoxicity of TQ-NLC as reflected by the IC50 value at various incubation times determined by using MTT assay.

Cell line	IC50 value (M)
	24 h	48 h	72 h
Cancerous cell line
MDA-MB-231	6.50 ± 0.50^a	4.43 ± 0.12^b	4.47 ± 0.06^b
MCF-7	>50	>50	>50
SiHa	19.42 ± 0.33^c	10.42 ± 0.17^d	8.50 ± 0.14^e
HeLa	23.00 ± 0.14^f	18.17 ± 0.51^g	15.58 ± 0.17^h
Normal cell line
3T3-L1	NP	NP	>50
Vero	NP	NP	32.00 ± 0.29^i

Values were the means of three replicate samples. The data were presented as mean ± SEM. a, b, c, d, e, f, g, h, and i were significantly different ().
NP = not performed.